The Seven commercial Egyptian wheat varieties : Misr1, Giza168, Sakha94, Sids1, Gemmiza7, Gemmiza11, and Shandawel1) were used to investigate the expression of defensin genes during seed germination. Three specific pairs of primers designed to amplify fragments of their respective defensin genes (PDF3, PDF5, and PDF30)were used. The reverse primers were utilized to synthesize cDNA from total RNA isolated from wheat shoots. PCR was used with cDNA to amplify fragments of defensins genes from the seven varieties. Results of this study showed that there was difference in defensin gene expression among the seven varieties. This included absence of PDF5 expression in Sids1and absence of PDF30 expression in Gemmiza7. Also, variations in the gene expression level among species were detected. Misr1 showed low expression, whereas Shandawel1 gave the highest expression of the three studied genes. Other varieties represented various degrees of expression for the three genes. It seemed that the difference in defensin gene expression would be related to the resistance of wheat varieties to diseases and a biotic stresses. This study would contribute information about defeinsin gene expression as a biodiversity tool in wheat breeding programs and variety evaluation.