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44030

NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI

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Last updated: 04 Jan 2025

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Abstract

Effect of some nutritional and environmental factors on growth and cellulase production by Bacillus alcalophilus S39 and Bacillus amyloliquefaciens C23 was investigated. Results indicated that 1% carboxymethylcellulose (CMC) and 0.7% yeast extract were most effective as the carbon and nitrogen sources respectively. Initial pH 7 and 3% inoculum size (3.5-4.3 ×106 CFU/ml) found to be optimal for growth and cellulase production. Incubation temperature at 30ºC and 45ºC achieved the highest activity of cellulase for Bacillus alcalophilus S39and Bacillus amyloliquefaciens C23 respectively, and the suitable shaking rate was 150 and 200 rpm.  

DOI

10.21608/jacb.2015.44030

Keywords

β-glucosidases, FPase, CMCase, B. alcalophilus, B. amyloliquefaciens

Authors

First Name

Khadiga

Last Name

Abou –Taleb

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Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Ain Shams Univ., P.O.Box 68, Hadayek Shoubra 11241, Cairo, Egypt.

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Orcid

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First Name

W.

Last Name

Mashhoor

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-

Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Ain Shams Univ., P.O.Box 68, Hadayek Shoubra 11241, Cairo, Egypt.

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Orcid

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First Name

Sohair

Last Name

Nasr

MiddleName

-

Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Ain Shams Univ., P.O.Box 68, Hadayek Shoubra 11241, Cairo, Egypt.

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-

City

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Orcid

-

First Name

M.

Last Name

Sharaf

MiddleName

-

Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Ain Shams Univ., P.O.Box 68, Hadayek Shoubra 11241, Cairo, Egypt.

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Volume

6

Article Issue

2

Related Issue

6875

Issue Date

2015-02-01

Receive Date

2015-02-03

Publish Date

2015-02-01

Page Start

29

Page End

39

Print ISSN

2090-3626

Online ISSN

2090-3707

Article File

JACB_Volume 6_Issue 2_Pages 29-39.pdf

PDF . 664.7kB

Link

https://jacb.journals.ekb.eg/article_44030.html

Detail API

https://jacb.journals.ekb.eg/service?article_code=44030

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

NUTRITIONAL AND ENVIRONMENTAL FACTORS AFFECTING CELLULASES ACTIVITY PRODUCED BY HIGH POTENT CELLULOLYTIC BACILLI

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Article

Created At

22 Jan 2023