Apot experiment was carried out at the Experimental Farm of Environ. & Bio-Agriculture Dept, Fac. Agric., Al-Azhar Univ., Nasr City, Cairo, Egypt , during the winter season of 2014 to study the impact of some plant growth promoting rhizobacteria (Bacillus circulans and Bacillus polymyxa),  EM and compost tea on growth, chemical composition and yield of organically cultivated spinach plants cv. balady. Results could be summarized as follows: All the treatments of "PGPR'' significantly increased the vegetative growth characters, i.e., plant height (cm), number of leaves, leaves area (cm²) fresh weights (g)/ P, dry weights (g)/P and  Leaves weight (g/pot)  over  the control plants. Results also indicated that the highest significant increase in yield (g/pot) was due to T9 (compost + B. polymyxa). Total nitrogen percentages of spinach plants reached the maximum to be 2.21, 2.14 and 2.10 with T3 (Compost + EM), T6 (compost + B. polymyxa) and T11 (Compost + EM+ Compost tea  + B. polymyxa), respectively. While, the highest total percentages of phosphorous and potassium were due to T9 (compost +                            B. polymyxa) and T12 compost + EM + compost tea + B. polymyxa + B. circulans).  The results also, indicated that Treatments 7 (Compost +EM+ Tea) and 12 (Compost + EM+ Compost tea + B. polymyxa + B. circulans)  were significantly in total chlorophyll content a higher  content than the control. The treatment 3 (compost + EM) achieved less values for Ca% (0.73%), oxalate (1.24%) and Ca oxalate (1.81%).  The treatment (Compost + EM+ Compost tea) gave the highest soil nitrogen content. While, T8 (Compost + EM+ Bacillus circulans) recorded the highest soil P content.