This study was carried out to investigate the effect of some combinations of two plant growth regulators on the multiplication and stability of the two strawberry genotypes, Sweet Charlie (SC.) and Florida (FL.) cultivars. Shoot tips of the two cultivars were cultured on MS medium supplemented with combinations of BA at 3.0 mg/l with three levels of GA₃at (0.2, 0.4, 0.6 mg/l). Data were collected after 8 weeks of cultured. The results showed that Sweet Charlie produced better multiplication than the Florida. DNA extracted from leaf tissue of in vitro derived plantletswere analyzed by RAPD-PCR to detect possible drift in genetic stability of micro-propagated plants.The results showed the different fingerprints of RAPD with concentrations of BA and GA₃ turn for variation within replicates per concentration. The results showed the highest polymorphism appeared in Florida when was compared to Sweet Charlie, the results indicate that the Sweet Charlie is more stable and would be more suitable for in vitro micro-propagation.