Twenty-two genotypes (parental strains) of sugarcane were chosen from the germplasm bank of Sugar Crops Research Institute (SCRI), Egypt to study diversity and relationship among sugarcane genotypes based on genetic profiles using 18 SSRs primer, twelve of them were sorghum specific primers. The genetic similarity ranged from 3 to 100 % among the 22 sugarcane genotypes. Most dissimilar of all the genotypes was Phil 8013 with genetic distance of 50 %. The cluster analysis classified the 22 sugarcane genotypes into eight groups. Analysis of different SSRs was useful tool for detection genetic diversity to enhance plant breeding programs. Applying SSRs is important specially in sugarcane that can be a troublesome process as a result of the complexness of sugarcane genome. It is often concluded that determine of genetic similarity based on SSRs could give a lot of accurate data to plant breeder. This information can facilitate the exploitation of sugarcane germplasm on molecular basis Future breeding efforts involving crosses within and among the groups identified during this investigation is also helpful for combining useful genes and alleles in new sugarcane genotypes in addition to maintaining genetic diversity