Twelve bacterial isolates were recovered from the rhizosphere of cotton, flax, and tomato seedlings of the most predominat commerical cultivars in Egypt. Bacterial isolates determined for their activities against three phytopathogenic fungi: Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii.When the six most antagonistic isolates were classified by standard tests, it was found that 4 strains (57 %) were belonging to Bacillus spp., 2 strains (40 %) were belonging to pseudomonas spp. In vitro,Bacillus subtilus (2strains),Bacillus sp. (2strains) , Pseudomonas flurorescens and Pseudomonas sp. were effective antagonists. Ammonia,chitinase, amylase and cellulase in bacterial culture by Bacillus sp., were produced and also, the production of siderosphore,ammonia, lipase and chitinase by Pseudomonas spp.may contribute to the antagonistic  activities of the bacterial isolates.