In this study, thirty two bacterial isolates were randomly isolated from  skin, wound and hair of patients. Antibiotic susceptibility of the collected bacterial isolates to seventeen antibiotics was studied using the disc diffusion method. Results revealed that bacterial isolates number (9) and (17) were proved to be the most multi-drug resistant isolates recording 100% resistance. Bacterial isolate number (9) was subjected to molecular identification by using 16S rRNA gene sequence, the tested bacterial isolate revealed a similarity of 98% with Staphylococcus aureus subsp. aureus JH1. The antibacterial activity of Saragassum latifolium, Ulva lactuca and Jania rubens were tested against the most multi-drug resistant bacterial isolate. Saragassum Latifolium extract appeared the highest antibacterial activity. Ultraviolet spectrum of some compounds is recorded in conjunction with other spectral data such as infrared and GC-MS analysis as an attempt to deduce the compounds which caused inhibition to the growth of bacterial isolate. The suggested chemical structure of purified antagonistic material isolated from Saragassum latifolium.