Bacteriological examination was carried on two types water samples were collected from River Nile water and ground water at different cities of  El gharbia government., Egypt during 2012 /2013 in order to determine the levels and total counts of bacterial isolates .During this study  about 60 bacterial isolates were present in our water collected  samples . These isolates were  tested on  different selective  bacterial media like nutrient agar ,Staph., Serratia ,Proteus , Pantoae  and  Aeromonas media  for determine the total counts of these genera. The highest number of total bacteria was found on summer in both types of water . After that  the number of different bacterial genera were presence on River Nile water while Proteus mirabilis , Serratia Plymuthica and Pantoea spp. were few on ground water .