Six fungal strains were isolated from the rhizosphere of sugar beet cultivars in Egypt. Two fungal isolates were effective against three phytopathogenic fungi: Fusarium solani, Fusarium oxysporum and Fusarium dimerum.The two strains were identified and belonged to Trichoderma viride. In vivo the highest percentages of survival plants were 72.5, 75 and 82.5%by T.viride codeNo. (2) for the control of F. solani, F. oxysporum and F. dimerum, respectively while more than T.viride codeNo. (1). T. viride isolates produced HCN, chitinase and cellulase, but it failed to produce IAA. The usage of T. viride as seed treatment reduced the percentage of damping-off of sugar beet under greenhouse conditions. This result shows that these T. viride are very effective biocontrol agents and should be harnessed for further biocontrol applications.