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38902

Extraction, Purification and Spectroscopic Characterization of Phycobiliproteins Extracted from some Nostoc Spp.

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Last updated: 22 Jan 2023

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Abstract

Phycocyanin and phycoerythrin are considered the major phycobiliprotein in many cyanobacteria as well as being a secondary phycobiliprotein in some red algae. In this article, the biomass of Nostoc linckia was harvested at the 16th day, which approximately equalled 361 mg/L dry biomass for attaining the maximum content of phycocyanin, and the biomass of Nostoc carneum was collected at the 17th day that approximately equalled 326 mg/L dry biomass for attaining the maximum content of phycoerythrin. Phycocyanin and phycoerythrin were extracted from both Nostoc linckia and Nostoc carneum respectively. They were extracted by successive cycles of freezing and thawing using 50 mM phosphate buffer solution (pH 7) then purified by single step of precipitation in 65 % of ammonium sulphate. Phycocyanin and phycoerythrin achieved 2.29 and 3.02 of purity ratio; respectively. The purified phycocyanin and phycoerythrin exhibited maximum absorbance at 614 and 560 nm respectively.

DOI

10.21608/jacb.2017.38902

Keywords

Nostoc spp. growth, phycobiliproteins, phycocyanin, phycoerythrin, Extraction, purification ratio, Characterization

Authors

First Name

Mervat

Last Name

Hussein

MiddleName

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Affiliation

Botany Dept., Fac. of Sci., Mansoura Univ., Mansoura, Egypt

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First Name

Noura

Last Name

El-Naggar

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Affiliation

Dept. of Bioprocess Development, Genetic Engin. and Biotechnol. Res. Inst.,, City of Scientific Res. and Technol. Appl., Alexandria, Egypt

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Orcid

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First Name

Asmaa

Last Name

El-Sawah

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Affiliation

Botany Dept., Fac. of Sci., Mansoura Univ., Mansoura, Egypt

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Volume

8

Article Issue

10

Related Issue

6235

Issue Date

2017-10-01

Receive Date

2017-10-18

Publish Date

2017-10-01

Page Start

261

Page End

264

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_38902.html

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https://jacb.journals.ekb.eg/service?article_code=38902

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4

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

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Article

Created At

22 Jan 2023