Seven teosinte inbred lines (four lines as females and three lines as male parents) derived through selection from segregating generation of three crosses were used in this investigation. They were utilized in a factorial mating design in the 2014 summer season at El-Serw Agricultural Research Station to produce 12 F₁ hybrids. The seven parental lines and their 12 F₁ hybrids were evaluated during 2015 and 2016 summer season using Randomized Complete Block Design with three replications. In this study, the amount of heterosis and nature of gene action were investigated for green fodder yield and its component traits. The results indicated that highly significant differences were detected among the studied genotypes. In addition, the mean squares of interactions with years were highly significant for all studied traits.  The P₄ had highest and desirable mean values for most studied traits. The hybrid P₃ ×P₅ showed the highest mean performances for most studied traits for three cuts. The amounts of heterosis over the mid-parent (H_M.P%) for green fodder yield per plant ranged from 5.77% for P₂ × P₅ at C₁ to 259.23% for P₁ × P₆ at C₃. The values of heterosis for dry fodder per plant ranged from 1.03% for P₂ × P₆ at C₁ to 145.68% for P₁ × P₆ at C₃. The inbred line P₆ was the good combiner for green fodder yield per plant and dry fodder yield per plant. The highest SCA effects were observed in the hybrid P₃× P₅ for most traits in the three cuts and could be promising cross improving green yield in teosinte breeding programs. The presence of large and significant estimates of additive and non-additive types of genetic variances indicated the importance of both types suggested the utilization of both types for ultimate improvement. The results showed that the phenotypic distances (PD) ranged from 479.3 to 2864.9 for parental lines and from 416.9 to 3716.7 for F₁ hybrids.