Sixty two isolates of actinomycetes were isolated from different eight sandy and clay soils from various locations of Egypt (Damietta and Mansoura) during the year 2016. All isolates of actinomycetes were screened against known different types of bacteria and fungi like: three Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and one fungus (Candida albicans).  About nineteen strains of actinomycetes were isolated which had different antibacterial and antifungal activity. Two potent actinomycetes isolates were selected to be identified according to their morphological, physiological and biochemical characterization and identified as Streptomyces rimosus.