The present study was conducted to isolate, identify and characterize the Streptomyces strain using morphological and biochemical analysis. This strain was molecular identified by sequencing the 16S rRNA gene as Streptomyces thinghirensis EGDA6S isolate. Results for scanning electron microscopy (SEM) revealed that the tested isolate is belonging to yellow series of Streptomycetes with smooth spore surface. Its cell wall contains LL-Diaminopimelic acid (LL-DAP) with non-characteristic sugar pattern and it does not produce melanin pigmentation. S. thinghirensis EGDA6S' metabolite revealed a considrable antimicrobial activity against some microorganisms namely: Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Whereas, the maximum antimicrobial activity was observed on oat meal followed by starch nitrate media. Maximum antimicrobial activity being 7 days, at 30^oC and pH 7, for optimum incubation period, temperature and pH.