The crude chloroform and methanol extracts of Lepidium sativum seeds belong to family Brassicaceae,were used in this work to study their efficiency to reduce Aflatoxin B₁ in rats. The more effective of chloroformic extract which decreased Aflatoxin B₁  level from 8.7 to 1.0 ppb at a dose of 300 mg/kg body weight comparing with ligalon (Silymarin) which reduced Aflatoxin B₁ to 0.97 ppb in concentration of 100 mg/kg after 5 weeks, followed by methanolic extract at a dose of 300 mg/kg body weight which showed the mean value of 1.2 ppb.Also, the more effective treatments may be Silymarin in concentration of 100 mg/kg followed by chloroformic extract, while methanolic extract of Lepidium sativum seeds was represented middle effect for blood tests  of rats injured with Aflatoxin B₁ e.g. Serum marker enzyme parameters (MDA, SOD, ALP and Bilirubin), kidney functions (Creatinine and Urea), liver functions (ALT, AST, Total proteins, Albumin and Globulins), lipid profile (triglycerides, total cholesterol, HDL-c, LDL-c and vLDL-c ) and haematological parameters (HB, RBCs, Plt and WBCs), were determined in injured rats comparing with control rats. It is quite clear that Silymarin is considered synthetic product, so the natural extracts of garden cress are more suitable for usage due to, they considered more safety and economic products, so these extracts can be additive to foodstuffs and therapeutic drugs owing to their multi medicinal benefits.