Beta
35227

Identification of Four Thermophilic Geobacillus Isolates from Hammam Pharaon, Sinai, Egypt

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

-

Abstract

  The present study was conducted to identify thermophilic bacteria from local habitats and to test their ability for producing thermostable α-Amylase, cellulase, lipase and protease in order to pave the way for their use in industrial processes. Out of 29 isolates initially obtained mainly from Hammam Pharaon, four potential isolates were selected based on thermo-tolerance ability and enzyme activity. The four isolates were characterized using phenotypic and 16S rRNA sequencing which recognized the isolates as Geobacillus. All four isolates were found to be variable for indole test, methyl red and rod shaped morphology. However, they were found to be variable for citrate utilization, catalase test starch hydrolysis, gelatin hydrolysis, casein hydrolysis, and Tween 80 hydrolysis. These bacteria grow at temperatures ranging from 50 to 90°C and the pH ranged from 6 to 9. This study added a lot to our knowledge about the thermophilic bacteria inhabiting Hammam Pharaon and their characters that would open the door for their use in biotechnological applications..  

DOI

10.21608/jacb.2018.35227

Keywords

α-Amylases, Cellulase, Protease, Lipase, thermophilic Geobacillussp, Hammam Pharaon

Authors

First Name

Y.

Last Name

Osman

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

Email

ellazeiky@yahoo.com

City

-

Orcid

-

First Name

A.

Last Name

Mowafy

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Abdelrazak

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

First Name

Amira

Last Name

El-Mallah

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

Email

-

City

-

Orcid

-

Volume

9

Article Issue

7

Related Issue

5809

Issue Date

2018-07-01

Receive Date

2018-07-01

Publish Date

2018-07-01

Page Start

151

Page End

157

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_35227.html

Detail API

https://jacb.journals.ekb.eg/service?article_code=35227

Order

1

Type

Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

Identification of Four Thermophilic Geobacillus Isolates from Hammam Pharaon, Sinai, Egypt

Details

Type

Article

Created At

22 Jan 2023