Pythium ultimum causal of damping –off in sugar beet, which is one of the most destructive diseases in this crop worldwide..During the study, twelve bacterial  isolates were  isolated from rhizosphere soil of  sugar beet crop. Three isolates of them showed antifungal activity against these phytopathogen. These isolates were identified as: Bacillus amyloliquefaciens , Bacillus pseudomycodies and Bacillus sp. by standard tests and the application of biolog system.Three species of fungi as Trichoderma spp.were successfully used by several investigators to control .In vivo, results of seeds soaking with tested B. amyloliquefaciens showed that the most effective in controlling damping –off disease(80%) followed by B. pseudomycodies (66.67%). While, T.  viride recorded value of survival plants (60%), T. hamatum and T.harzianum (53.33%, 23.33%, respectively).At the same time  seeds coating with T.harzianum was the most effective in controlling disease indicated that  (86.67%), followed by B. pseudomycodies , B. amyloliquefaciens , T. viride , Bacillus sp.and T. hamatum(80.00%, 73.33%, 50.00%, 46.67%, 40.00 % respectively) in soil infested with P. ultimum.