After a single intravenous (IV) and intramuscular (IM) injection in broiler chickens, the disposition kinetic profile of cefotaxime was explored. Cefotaxime was administered at 10 mg / kg b.wt dose level for both routes. The serum cefotaxime concentrations estimated at 0.08 h were 47.06 μg / ml after IV injection, which gradually decreased and cefotaxime was identified up to 12 h (0.88 μg / ml). In broiler chickens, the average values of Cl_tot, Vd_ss and T_{0.5 β}of cefotaxime were 0.09 L kg^-1 h^-1, 0.38 L kg^-1and 2.49 h. The highest serum concentration (C_max) after IM injection was (22.21 ± 2.03 μg / ml), the maximum serum concentration period (t_max) was (1.16 ± 0.11h) and the half-life of elimination (T_{0.5 el}) was (3.24 ± 0.31h). Bioavailability after IM injection was 84.27%, and in vitro protein binding percent was 28.79 %. A recommended IM dosage for cefotaxime in broiler chickens would be 10 mg / kg b.wt., administered intramuscularly at 12 h intervals, providing a therapeutic serum concentration in broiler chickens exceeding the MIC ≤ 0.5 μg/ml for most sensitive bacterial pathogens in broiler chickens.