The incidence of encysted metacercaria (EMC) was investigated in 200 freshwater fish samples represented 100 from each of Clarias gariepinus and Cyprinus carpio. Fish were collected from River Nile resources at EL-Daqahlyia governorate. The total incidence of EMC in examining fish was 73.5%, while in Clarias gariepinus and Cyprinus carpio were 86% and 61%, respectively. An artificial digestion technique for collection and identification of encysted metacercariae from investigating infected fish revealed that EMC is belonging to Prohemistominae in Clarias gariepinus and Heterophyidae in Cyprinus carpio. The experimental infection of puppies with isolated EMC from the studied freshwater fishes revealed the detection of four trematodes species: Prohemistomum vivax, Mesostephanus appendiculatus, Heterophyes heterophyes, Procervim varium. The pathological changes due to the effect of the developmental larval stages (EMC) and their adult zoonotic trematodes on their hosts were studied. Due to health hazards and impact of such these fish born zoonotic trematodes (FBZTs), this study focuses on the validation of the traditional use of Moringa oleifera derivatives for exterminate of intestinal helminths as the obtained result revealed that Moringa oleifera seed oil have higher anthelmintic activity than Moringa oleifera leaf extract.