Through this recent study sonication of the previously sporulated Eimeria acervulina oocysts were used for immunization purpose. A total number of 80 susceptible chicks were divided into four groups (A, B, C and D, 20 chicks each), the first three groups received sonicated sporulated oocysts of Eimeria acervulina in dose of 30000, 40000 and 50000 per so /one chick respectively while group D was left without immunization as a control group. The first three groups withstand challenge test using hot strain of Eimeria acervulina and showed protection percentage of 70, 80 and 85% respectively with minimal lesion score, obvious increase body weight gain and good health condition compared with the control group which recorded a high level of score lesion up to +4 with 0.0% protection and a high mortality rate. Also observed reduction in fecal oocysts shedding in immunized chicks compared with the control one. On applying ELISA test for confirmation it was found that there was increasing in antibody titer against Eimeria acervulina in immunized birds compared with those of the control group.