Eighty three slaughtered donkeys (Equus asinus), 3-33 years old at Giza Zoo slaughter house, were examined for the presence of hydatid cyst from May 2014 to December 2015. Seventeen donkeys out of 83 (20%) had hydatid cysts in their livers. Clinical signs were almost asymptomatic. Different sized thick-walled hydatid cysts, each 6–8 cm in diameter in the liver. Incised cysts contained a turbid, amber liquid mixed with hydatid sand.  Fertility of the cysts was assessed by examining the cysts' fluid for the presence of protoscoleces and the viability of the protoscoleces was determined using staining with an aqueous solution of 0.1% eosin stain. Seven out of 17 examined cyst (41%) were fertile where 4 out of 7(57%) were viable. Prevalence and intensity of infection increased with age. Microscopically, the tri-layered cyst walls consisted of an outer adventitial layer, a laminated acellular intermediate layer, and an inner germinal membrane. The result  revealed hepatic hydatidosis in 20% of donkeys indicated the contamination of the environment in Egypt with Echinococcus granuloses.