Apigenin and gallic acid are naturally occurring plant flavonoids. They have antioxidant, anti-inflammatory, antitrypanosomal, and antimalarial activities. In this study, the restrictive properties of Apigenin and gallic acid were evaluated against two Babesia species and Theileria equi in vitro.  Apigenin was evaluated against B. microti in mice. Apigenin showed significant growth inhibition for Babesia bovis, Babesia caballi, and Theileria equi with IC₅₀ values of 125 µM, 60 µM, and 0.08 µM, respectively. IC₅₀ values of gallic acid were 30, 30, and 4.5 µM for Babesia bovis, Babesia caballi, and Theileria equi, respectively.  Apigenin at a dose rate of 5 mg/kg resulted in a 65 % restriction of Babesia microti progression in BALB/c mice. Apigenin may be a promising drug therapy in bovine and equine piroplasmosis.