This study aimed to evaluate 2-months pre- and 3- months' post-partum dietary supplementation of Aspergillus oryzae (AO) extract (Amaferm®) or malate salt (MS) on productive performance of buffalo cows. Multiparous buffalo cows (n=15, 446.92±26.54 kg LBW, and 2-5 parities) at late pregnancy period were divided into three groups., The control group was fed a basal ration, while the 2^nd and 3^rd groups received the basal diet with 15 g/h/d of AO and 10 g/h/d MS, respectively. Results indicated that AO supplementation improved (P<0.05) DM and OM digestibility during post-partum, while CF digestion, feeding values, and ruminal TVFA's concentration were increased (P<0.05) by MS and AO. Treated groups increased (P<0.05) milk yield and composition, MS group recorded the highest benefits. AO addition improved (P<0.05) milk production persistency during lactation period. Economic efficiency had increased (P<0.05) for AO and MS groups by 4.6 and 13.3% compared with the control, respectively. Dietary AO supplementation (15 g/h/d) 2 months pre- and 3-months post-partum improve nutrient utilization and productive performance of multiparous buffalo cows.