In vitro comparative efficacy studies on the addition of fix-a-tox, antitox plus, tafla, autoclaving, microwave, hydrogen peroxide, ammonia solution and extraction with aqueous methanol (50%) as detoxifying agents to a diet containing 3600 ppb or 1800 ppb total aflatoxins.  The 0.5% level of antitox plus, fix-a-tox and tafla gave the most constant pattern with aflatoxin reduction of 33.4, 16.7 and 44.4%, respectively at the high level of toxin but at the low level the reduction was 31.6, 15.8 and 42.1%, respectively.  The autoclaving for 30 min and using microwave oven at a medium energy for 3 min gave reduction of 33.4 and 52.8% at the high level of toxin but at the low level of toxin the reduction was 31.6 and 51.6%, respectively.  The effect of high temperature on the appearance, consistency and composition of a food (consequently its biological value, digestibility and utilization) raises special attention to the choice of protection as the corner stone of the aflatoxin control which is more beneficial than detoxification.  Many chemicals have been tested for their ability to structurally degrade or inactivate aflatoxin, including H₂O₂ and ammoniation.  These chemicals resulted in a reduction of 38.9 and 88.9% at the high level of toxin but in the low level contaminated diet, the reduction was 36.8 and 87.9%, respectively.  Extraction of aflatoxin from the diet with methanol caused a reduction of 55.6% at the high level of toxin but in the low level contaminated diet, the reduction was 53.2%.  However, this method for the removal of aflatoxins via solvent extraction appears to be impractical and expensive when compared with other methods. There was no difference in the chemical composition for all different treatments, yet the treatment with ammonia increased crude protein (CP)% but the extraction caused a decrease in CP% and hence in the nutritional quality. Aflatoxin productivity from Aspergillus flavus in liquid yeast media in the absence and presence of 0.5% of each of antitox plus, fix-a-tox, tafla, 1% of ammonia and 3% level of H₂O₂ was determined in the samples. Addition of any of these agents resulted in a decrease in aflatoxin productivity with the maximum effect for ammonia followed by H₂O₂, tafla, antitox plus and fix-a-toxin.  Ammonia prevented the growth of Aspergillus flavus and this resulted in prevention of aflatoxin contamination.