Serum total proteins, albumin, globulin, A/G ratio, alkaline phosphatase (AP) aspartate transaminase (AST) alanine transaminase (ALT), cholesterol and total lipids were estimated in 23 multiparous Egyptian buffaloes aged 5-11 years from 60^th     days prepartum to 90^th day postpartum at 15 days intervals. Total proteins and globulin were high at 60^th day prepartum, then decreased gradually until parturition (P< 0.05) but increased again after parturition with fluctuation until 90^th day postpartum. Albumin was slightly fluctuated throughout the experimental period. Albumin/gbbulin ratio was low at 60^th day prepartum, then increased prior parturition until 90^th day postpartum. Concentration of AP was high at 60^th day prepartum then decreased towards parturition, statistically non-significant. AST, ALT, cholesterol and total lipids decreased (P< 0.01) from 60 days prepartum until parturition where they increased (P<0.05). The same parameters did not differ between the two groups. However, buffaloes which exhibited estrus (group1) showed a tendency to higher 0levels for all parameters than buffaloes which did not exhibit estrus (group2).