To examine the role of oxytocin in eliciting milk ejection, an experiment was conducted at El-Serw farm belonging to Animal Production Research Institute, Ministry of Agriculture. A total of 24 Baladi cows were divided into three equal symmetric groups and injected intramuscularly prior milking with either saline (control) or 5 IU and 10 IU oxytocin.                 Administration of 10 IU of oxytocin produced shortest (P<0.05) milk let down time and milking time (2.0 and 4.2 min, respectively), also greatest (P<0.05) peak flow rate and average flow rate (1.0 and 0.62 kg/min, respectively).                 Variations in stripping time (0.43 to 0.49 min) and in milk yield (2.2 to 2.7 kg/milking) were not significant, but more fat yield was harvested after administration of oxytocin (72.2, 95.3 and 94.4 g fat/milking for control, 5 IU and 10 IU oxytocin, respectively).                 Normal nutritive constituents of milk (g/100ml), specific gravity and somatic cell count were not significantly affected by oxytocin injection. There were no significant effects of oxytocin administration on total plasma protein, GOT and GýPT, creatinine and bilirubin and cortisol levels. These findings indicate that oxytocin enhances milk ejection reflex without direct stimulatory action on mammary and body metabolism.