Alteration of the biophysical and biochemical characteristics of cervical mucus (CM) due to differences in steroid hormones through the estrus cycle leads to different pregnancy rates. This investigation aims to study the possible relationship between CM properties with biochemical profiles, macro-mineral levels, and steroid hormones concentrations, in reference with pregnancy rates of Egyptian Baladi cows. Fourteen Baladi cows were used and synchronized. The model log-likelihood ratio was highly significant (P=0.0009), and reported that the spinnbarkeit (SPK), electrical conductivity (EC), and crystallization (CRS) had significant effect on pregnancy rate. The 3^rd level of SPK (>13.5 cm) and EC (>15 mS/cm) showed significantly the highest effect (P=0.0016 & 0.0517, respectively) and a clear positive of estimate marginal effect (20.2543 & 10.6192, respectively) attitude towards the pregnancy rate. However, in case of the CRS, the significant effect was in the first two levels (P=0.0321 & 0.0425, respectively) with pregnancy rate, reverse the last 2 levels. Total proteins, cholesterol, glucose, potassium, and chloride levels, and estradiol concentrations were increased with increasing levels of SPK and EC, and appearance of typical fern patterns (first two levels of CRS), in contrast to sodium level and progesterone (P4) concentrations that decreased with elevating levels of SPK and EC and appearance of atypical fern patterns (last two levels of CRS). There was a close correlation between CM properties and steroid hormones (P₄ & E₂). So, alterations in CM properties, especially SPK, EC, and CRS, can be utilized to foresee estrus time and, as a result, insemination time.