249792

Effect of Aqueous and Methanolic Green Tea Extracts on Motility, Normal Forms, And Kinetic Parameters of Spermatozoa in Rahmani Ram Semen Equilibrated at 5 °C for 4 Hours

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Last updated: 04 Jan 2025

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Abstract

Green tea extract is plant–derived natural antioxidant. The purpose of this study was to investigate the effect of the supplementing semen extender (Tris-citric-soybean lecithin) with different levels of aqueous (AGTE) or methanolic (MGTE) green tea extract (0.5, 1.0 and 1.5%) on the motility, livability, normality, plasma membrane integrity, and kinetic parameters of ram spermatozoa in diluted and equilibrated semen. Semen was collected from 5 rams once a week for 7 weeks using an artificial vagina. Results showed positive effect of extender containing 1% MGTE on visual motility, livability, abnormality, membrane integrity, rapid motility, sperm velocity, and normal form in post-equilibrated semen. It is possible to infer that the inclusion of methanolic green tea extract at a level of 1% in semen extender had a substantial effect on sperm quality, normality, function, and velocity of ram spermatozoa in Rahmani semen after dilution and equilibration for 4 h at 5oC.

DOI

10.21608/jappmu.2022.145028.1041

Keywords

Aqueous and Methanolic green tea extract, ram spermatozoa, equilibration, motility CASA analysis

Authors

First Name

M.

Last Name

Abdellatif

MiddleName

A.

Affiliation

Animal Production Department, Faculty of Agriculture, Tanta University, Egypt.

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-

First Name

S.

Last Name

Gabr

MiddleName

A.

Affiliation

Animal Production Department, Faculty of Agriculture, Tanta University, Egypt.

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City

-

Orcid

-

First Name

M.

Last Name

El-Sherbieny

MiddleName

A.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

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City

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Orcid

-

First Name

A.

Last Name

Yousif

MiddleName

I.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Cairo, Egypt.

Email

ahmedyousif2788@yahoo.com

City

Kafr El-shiekh

Orcid

0000-0003-0770-9680

Volume

13

Article Issue

5

Related Issue

35244

Issue Date

2022-05-01

Receive Date

2022-07-19

Publish Date

2022-05-01

Page Start

63

Page End

70

Print ISSN

2090-3642

Online ISSN

2090-3723

Link

https://jappmu.journals.ekb.eg/article_249792.html

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https://jappmu.journals.ekb.eg/service?article_code=249792

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2

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Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

MainTitle

Effect of Aqueous and Methanolic Green Tea Extracts on Motility, Normal Forms, And Kinetic Parameters of Spermatozoa in Rahmani Ram Semen Equilibrated at 5 °C for 4 Hours

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Article

Created At

22 Jan 2023