This experiment was conducted to study the drastic effects of graded levels
of atlatoxin-B, on clinical symptoms, residual effects, some blood constituents and
histopathological changes in Nile tilapia, O. nifoticus fingerlings. Also, it was conducted for experimenting the inhibiting effects of graded
levels of natural, cheap and available two adsorbent agent, namely egg shells(ES)
and shrimp wastes(SW) against the adverse effects of aflatoxin-B, contamination of
fingerlings diet fed for 8 weeks. The effects of aflatoxin-B1 (AFB1) were severe clinical lesions in the external
organs and postmortem symptoms in the internal organs of the aflatoxicated fish.
Significant increase in organs indices was calculated. But decrease in dray matter
and increase in ether extract of the fish liver were estimated. Significant decrease in
hemoglobin concentration, red blood cells count and uric acid and significantly
increase in white blood cells count and alkaline phosphatase and transaminases
activity were determined. Residues of AFB1 were found in the whole body of the
aflatoxicated fish directly at the end of the experiment and tended to decrease after
freezing periods. Severe histological alterations were recorded in livers, kidneys,
intestines and gills of the aflatoxicated fish. These alterations in all tested organs were increased by increasing level of
aflatoxin (200 ppb AFB₁).The effects of either adsorbents at levels of 1 and 2%,
respectively were useful in reducing the toxic effects of AFB1 on fish, where it
increased significantly the hemoglobin concentration, red blood cells count and uric
acid in fish blood. Yet, it decreased significantly white blood cells count, alkaline
phosphatase and transaminases activity and most of the organs indices. However, these adsorbents (at levels of 1 % ES and 2% SW) alleviated the
toxic effects of AFB, on liver composition (OM and EE) of the fish .. Moreover, using
these adsorbents at these levels (1% ES and 2% SW) alleviated the adverse effects
of AFB1 on the histopathological changes in different internal organs (livers, kidneys,
intestines, and gills) of the aflatoxicated fish.