The present study was designed to evaluate the protective role of vitamin E
&. selenium (Se). vitamin A, vitamin C, and choline and their combination against the
harmful histological effects of anatoxin in rats. Adurt male albino rats, weighing 80·120
g were divided into similar groups. Rats fed 3 anatoxin doses being 5, 10 and 20
mg/Kg diet (3 positive control groups). Aflatoxin plus vitamin E (0.5 mg/kg diet) e. Se
(S~g/I drinking water), vitamin C (2 g/kg diet), vitamin A (500 m[U Ikg diet), choline (3
g/kg diet) and vitamins combination with choline at the same levels mentioned belore.
Each one from the live pretreatment groups is subdivided into three subgroups
according to doses of anatoxin (treated groups). The negative control group received
normal basal diet. Rats received aftatoxins with or without vitamins for 60 days then
scarified and liver and kidney were taken for histopathological examination. The histopathological examination of liver of rats treated by aflatoxins
revealed vascular dilatation, congestion, edema, hepatic cords disorganization,
aggregation of infiammatory cells, pyknotic nuclei and at the highest aflatoxins dose
revealed focal necrosis, destruction or bile duct epithelial cells and pleomorphic and
necrotic nuclei. The lesions showed increase in severity in a dose dependent manner,
However, kidney revealed damaged glomeruli, cloudy swelling in epithelial lining
tubules, interstitial hemorrhage and cellular infiltration, beside prominent degeneration
in renal tubules and glomeruli at the high doses. The lesions were dose dependent. The present study suggested that, administration of vitamins and choline
resul\w in reducing the toxic effects of afta\oxins on liver and Kidneys, The best
improvements in liver and kidney tissues were shown in groups treated by vitamin E +
Se and groups treated by the antioxidants mixture,