The removal of lead as heavy metal from polluted water by alginate beads of both immobilized live and heat killed fungal mycelia of Pleurerus sajur—caju and Ca alginate beads were investigated to determine the effect of treated and untreated water on laying Japanese quails performance . A hundred of 13 weeks old laying Japanese quails were divided into five groups. each group contained 20 birds divided into two replicates (10 hens for each ) . Group 1 (Tl) was allowed to drink water treated by immobilized live fungal mycelia . group 2 (T2) allowed to drink water treated by immobilized heat killed fungal mycelia . group 3 (T3) allowed to drink water treated by calcium alginate . group4 (T4) allowed to drink water contaminated by 0.3025 mg/L lead acetate and groups (T5) allowed to drink tap water (control) . The treatments were continued for 4 weeks after which all groups allowed to drink tap water for another 4 weeks. Lead acetate at concentration 0.3025 mg/L was added to water and the polluted water was treated by alginate beads of both immobilized live and heat killed fungal mycelia of Pfeurotus sajur—caju and calcium alginate beads. The concentration of lead in water after treatments decreased to 0.0082. 0.0014 and 0.008 mg/L when the water treated by alginate beads of both immobilized live and heat killed fungal mycelia of Pieurotus sajur-caju and Ca alginate beads respectively.. The results indicated that water contaminated by lead acetate increased lead concentration in liver at T4 compared with other treatments .The treatments had insignificant effects on eggs quality parameter. plasma total protein. albumin (A). globulin (G) and NG ratio while the treatments had significant effect on GPT and GOT. total cholesterol and creatinine , also the weight of spleen decreased insignificantly due to T4 treatment.