Total of 120 pullets from each strain Mandarah and Silver Montazah were used to study the effect of copper sulphate and worm-wood on decrease yolk and meat cholesterol concentrationin laying hens. Birds were randomly assigned to 3 groups according to dietary additives: group {1) was supplemented with 250 mg copper as CuSol.H207kg diet, group (2) was supplemented with 2% worm-wood and group (3) was fed basal diet as control. Water and feed were provided ad librtum. After 6 weeks of treatment (24-32) weeks of age, additives were removed and hens were switched to basal diet for 4 weeks (32-36) weeks of age. Supplementing 250 mg copper to diet for 6 weeks. significantly decreased plasma cholesterol by 36.0% and 30.7% for Mandarah and Silver Montazah. respectivelywhile the depression was 42.4% and 26.3% in both strains. when fed 2% worm-wood, respectively. Reduction in yolk cholesterol was 19.2% and 25.3% in Mandarah and Silver Montazah at 32 weeks of age by adding dietary copper. But yolk cholesterol depression was 22.2 and 30.1% in both strains by using 2% worm-wood in diet, respectively. Copper addition resulted reduction of 77.0 and 60.3% in breast muscle cholesterol and 73.6 and 81.1% in thigh muscle cholesterol for Mandarah and Silver Montazah, respectively. The corresponding depressions by dietary worm-wood were 74.3, 79.0 and 77.9 and 31.0%, respectively. Cholesterol concentration was much higher by about 22-24% in thigh than breast muscle Cholesterol level was more by about 4 fold in skin than muscles. Cholesterol depression by dietary additives was more by about 2 fold "1 muscle than breast in thigh.