Three feeding regiments for broilers were used in the present work, i.e. plant protein diet, (PPD). , PPD supplemented with either fish meal (PPDF) or meat and bone meal (PPDM). The Three regiments were enriched with vegetable oil (VO) or poultry fat (PF). Fatty acids content in breast, thigh and skin were determined. Total cholesterol, LDL and HDL cholesterol in blood serum breast and thigh meat was determined. Birds fed on feeds supplemented with PF had higher content of myristic and palmitic acids than those supplemented with VO. Also these birds had the highest content of cholesterol. Cholesterol content of breast and thigh for treatments fed PPD were the lowest compared to those fed on PPDF or PPDM. It is recommended to use vegetable oil in combination with PPD in raising broiler chicks to be sure that poultry meat (breast or thigh) had the lowest cholesterol level and suitable fatty acid profile.