Semen of 10 healthy mature crossbred (½ FR) rams was frozen in Tris-egg yolk glycerol extender before and after filtration through sephadex column G25-150 to investigate the effect of filtration on freezability and fertility outcomes during different seasons of the year. Filtration of semen through sephadex column resulted in significantly (P < 0.05) better maintenance of sperm progressive motility, live sperm count and sperm morphology compared to unfiltrated semen. There was significant (P < 0.05) decrease in sperm cell concentration x 10⁹/ml after filtration (2.05 + 0.5) over the unfiltered semen (2.68 + 0.08). Progressive motility of filtered frozen-thawed spermatozoa was significantly (P < 0.05) improved to progressive motility of unfiltered frozen semen (60.10 v.s 43.88%). Conception rate was higher for ewes inseminated cervically with filtered frozen-thawed semen (63.4%) than those inseminated with unfiltered frozen semen (52.3%) at synchronized estrus with GnRH-PG-GnRH protocol. However, conception rate at observed estrus for ewes inseminated cervically with filtered frozen semen was (P < 0.05) better in comparison with those inseminated with unfiltered frozen semen (95 v.s 75%). These results concluded that removal of dead and abnormal spermatozoa by filtration of ram semen through sephadex column resulting in highest fertility after cervical artificial insemination.