The present study was conducted to investigate the productive and reproductive performance of two local laying hen strains (Gimmizah and Mamourah) fed diets supplementation diets with yeast preparation.  Three hundred laying hens (150  hens Gimmizah and 150 hens Mamourah strain) 28 week-old were distributed into two groups. Each experiment at group were divided into five sub-group, the 1^st group fed on commercial layer diet as a control basal diet, the 2^nd group fed on basal diet +1 kg bio-buds (BB) / ton, the 3^rd group fed on basal diet +2 kg bio-buds (BB) / ton, the 4^th group fed on basal diet +1 kg yeast (Y) / ton and 5^th group fed on basal diet +2 kg yeast (Y) / ton. The results indicated that, supplementation Gimmizah hens diets with yeast preparation (BB or Y) at levels of (1 and 2 mg/kg diet) improved significantly daily feed intake (FI), feed conversion (FC), egg number (EN), egg weight (EW), egg mass (EM), Shell weight, yolk index, yolk color, Hough units and yolk cholesterol (mg/gm yolk). Concerning the dietary yeast levels, shell weight (%) were heaver significantly with layer hen strains feed diet supplemented with Bio-buds (BB) than in the yeast (Y). Feed intake and feed conversion layers fed on the diets content (Y) a significantly higher compared to those fed diets content (BB), the daily feed intake values of layers significantly decreased due to increasing dietary yeast levels. Significant improvement was recorded in EN with the increase in dietary yeast level. Supplementation Mamourah hens diets contain (BB) laid   significantly (P≤0.05) effect on EN, EW and EM in which the BB more egg than those fed diets contain (Y) While, the worst value recorded by layers fed the 0mg/kg level in BB or Y with Gimmizah strain. Shell thickens (mmx100), yolk color and Hough unit were significantly affected by layer hen strains.     In conclusion, the results of the present study indicated that, adding (Bio Buds) and (yeast) on commercial diets for Gimmizah and Mamourah strain may improve the productive and reproductive performance.