In endeavor to relieve the walking stress burden on Hassani goats during summer and winter seasons in El- Shalateen- Halaieb- Abo Ramad triangle. Probiotics (10g/h/d, Biovet - YC) were supplemented to the goats' diet. Twenty male Hassani goats, from the experimental flock of the Hedrba station belonging to DesertResearchCenter, were subjected to walk 10 km in 4 hours daily. The walking stress trial lasted for four days. Ten Hassani goats were fed a diet supplemented with probiotics for 28 days before and during the walking trials followed by 2 days for recovery. Meteorological data in terms of ambient temperature (AT) and relative humidity (RH) were recorded at 08.00 and 14.00 hrs. Additionally, thermo- cardio- respiratory responses in terms of rectal (RT), skin (ST) temperatures, respiration (RR) and heart (HR) rates were daily recorded before and after the walking trial. Likewise, hematocrit percentage (Ht), hemoglobin concentration (Hb), erythrocytes count (RBC's) were determined in blood and mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) were calculated. The results indicated that summer season had significant (P<0.01) higher values of RT, ST, Hb, PCV, RBC's and MCV and non-significant higher values of RR and HR than those of winter season. However, the values of MCH and MCHC in winter exceeded significantly (P<0.01) those in summer. Concerning the walking stress effects, the results revealed significant increments in the mean values of thermo- cardio- respiratory responses, PCV, MCV, MCHC (P<0.01) and Hb (P<0.05) but non- significant decrease in MCH value.     Probiotics supplementation during walking reduced significantly the mean values of RT, RR (P<0.05) and ST (P<0.01) while a non-significantly reduction was observed in HR value.  Moreover, probiotics caused significant (P<0.01) increases in Hb and Ht values and non-significant increases in RBC's, MCV and MCH. However, there was a non- significant reduction in MCHC values. It could be concluded that probiotics supplementation for walking stressed goats during summer and winter seasons might be an avenue to minimize the walking perils facing the Hassani goats raised under the harsh conditions of the El- Shalateen- Halaieb- Abo Ramad triangle.