The present study aimed investigate the effect of age on the physiological status of the rabbit bucks and to determine if the antioxidant properties of melatonin can protect aged rabbit males from oxidation stress. Forty five mature New Zealand White (NZW) rabbit bucks (15, 1 year-old and 30, 4 years-old) were used in the present work.  They were classified into three groups each of 15 bucks (a control and two treatments; T1 and T2). The control group included: mature bucks without melatonin supplementation (Adult), while T1 and T2 included aged bucks without melatonin supplementation (Aged), and aged bucks supplemented with 25 mg melatonin/kg for 8 weeks (Aged + Mel), respectively. Semen quality, some blood hematology, blood and seminal biochemical plasma in two ages of rabbit bucks, and the effect of melatonin supplementation on these traits were studied. The achieved results showed that most of the studied traits were adversely affected in aged rabbit bucks without melatonin supplementation. On the other hand Melatonin supplementation to aged males for 8 weeks, significantly increased sperm concentration, sperm motility, WBCs, blood and seminal plasma of GST and SOD. Also, melatonin supplementation decreased abnormal and dead sperms, as well as blood and seminal plasma of TBARS. In conclusion, it could be recommended that melatonin supplementation at a level of 25 mg/kg body weight to aged rabbit bucks was effective in minimizing the oxidative stress which was induced with the advance of age, specially for semen quality parameters, blood hematology, blood plasma of cholesterol and triglyceride, and blood and seminal plasma of  TBARS, GST, and SOD.