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202399

Characteristics, Yield, Flow Cytometer, DNA Fragmentation, and Comet Assay Parameters of Goat Spermatozoa in Semen of Zaraibi and Baladi Bucks at Young and Old Ages

Article

Last updated: 04 Jan 2025

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Abstract

The aim of this study was to determine goat breed (Zaraibi and Baladi) or buck age (young and old) effect on characteristics, production, flow cytometer, DNA fragmentation and comet assay parameters of spermatozoa. Twelve Baladi (BG) and Zaraibi (ZG) bucks, 3 old (2-4 years) and 3 young (8-12 months) from BG or ZG used for semen collection for 24 weeks. Volume (EV), sperm progressive motility (PM), livability (LS), abnormality (SA), and concentration (SCC) were evaluated, sperm outputs (SO) were calculated. Sperm apoptosis, DNA fragmentation and comet assay were analyzed. Results revealed that PM, LS, SA, SCC, total, motile, live, normal, and functional SO per ejaculate were significantly higher in ZG than BG and in old than young bucks in each breed. Both EV and LS were significantly higher, while SCC was significantly lower in old than young ZG. Each of EV, PM, and SCC was significantly higher, while SA was significantly lower in old than young BG. Viable sperm, haploid and cell cycle percent was significantly higher, while early apoptotic, apoptotic, and necrotic spermatozoa percent was significantly lower in old than young bucks of each breed. Spermatid percent in ZG and diploid percent in BG were significantly higher in old than young bucks. Tailed sperm, tail length, tail DNA percentage, and tail moment were significantly lower in ZG than BG, and in old than young bucks of each breed. It can be stated that Zaraibi bucks has a good potential for semen production than Baladi breed.

DOI

10.21608/jappmu.2021.95942.1021

Keywords

Semen, goats, breed differences, sperm function, apoptosis, DNA damage

Authors

First Name

Helmy

Last Name

Zaghloul

MiddleName

kotb

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

Email

helmyzaghlool@yahoo.com

City

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Orcid

-

First Name

M.

Last Name

El-Maghraby

MiddleName

M.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

Email

-

City

-

Orcid

-

First Name

A.

Last Name

El badawy

MiddleName

A.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Yousif

MiddleName

I. A.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

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City

-

Orcid

-

Volume

12

Article Issue

9

Related Issue

28519

Issue Date

2021-09-01

Receive Date

2021-11-01

Publish Date

2021-09-01

Page Start

305

Page End

314

Print ISSN

2090-3642

Online ISSN

2090-3723

Link

https://jappmu.journals.ekb.eg/article_202399.html

Detail API

https://jappmu.journals.ekb.eg/service?article_code=202399

Order

3

Type

Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

MainTitle

Characteristics, Yield, Flow Cytometer, DNA Fragmentation, and Comet Assay Parameters of Goat Spermatozoa in Semen of Zaraibi and Baladi Bucks at Young and Old Ages

Details

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Article

Created At

22 Jan 2023