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197370

Impact of Glucose Solutions in Tris-Based Extender on Sperm Variables in Turkey Semen Preserved at Cool Temperature for 96 Hours

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Last updated: 04 Jan 2025

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Abstract

This work aimed to study the effects of extenders containing sugar (300- and 370-mM glucose) on the storability and fertility of turkey semen. Semen was collected twice a week from 12 Bronze turkey toms of 40 wk old by abdominal massage for 4 wk. The collected semen of toms was pooled, mixed and extended at a rate of 1:2 with saline (E1), tris-eggyolk-300 mM glucose (E2), and tris-eggyolk-370 mM glucose (E3). After dilution, semen was stored for 96 hours at cool temperature (4-5oC). Semen was evaluated for motility, normality, and kinetic parameters of spermatozoa, then sperm fertility was determined for semen stored for appropriate storage time for each extender. Results show that overall mean of all sperm motility variables (total motility, total progressive, rapid and slow progressive, and non-progressive motility, and immotile spermatozoa), sperm-morphological parameters (head and neck abnormalities, mono and dual deformations, and sperm deformity index, SDI), and sperm-dynamic parameters (VCL, VSL, VAP linearity, straightness, and wobble) were improved by E2 and E3 as compared to E1. The fertility rate was higher (89.5%) for eggs produced from hens inseminated with E3-semen stored for 72 h (P<0.05) as compared to E1-semen stored for 2 h (77.5%), but E2-semen (85.83%) showed insignificant differences with both E1 and E3. Semen of toms could be stored with maintaining sperm function and fertility in tris-glucose-based extender for 72 h

DOI

10.21608/jappmu.2021.89927.1019

Keywords

Turkey semen, glucose-extenders, sperm motility, sperm normality, sperm kinetics, fertility

Authors

First Name

A.

Last Name

Abdel-Khalek,

MiddleName

E.

Affiliation

Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt.

Email

abdelkhalk2004@yahoo.com

City

-

Orcid

0000-0002-7839-2353

First Name

Samya

Last Name

Ibraheim

MiddleName

E.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

Email

isamya80@gmail.com

City

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Orcid

-

First Name

M.

Last Name

El-Sherbieny

MiddleName

A.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

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City

-

Orcid

-

First Name

Kh.

Last Name

Roushdy

MiddleName

-

Affiliation

-

Email

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City

-

Orcid

-

First Name

G.

Last Name

Younan

MiddleName

E.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

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-

City

-

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-

Volume

12

Article Issue

8

Related Issue

27933

Issue Date

2021-08-01

Receive Date

2021-10-02

Publish Date

2021-08-01

Page Start

241

Page End

249

Print ISSN

2090-3642

Online ISSN

2090-3723

Link

https://jappmu.journals.ekb.eg/article_197370.html

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https://jappmu.journals.ekb.eg/service?article_code=197370

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Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

MainTitle

Impact of Glucose Solutions in Tris-Based Extender on Sperm Variables in Turkey Semen Preserved at Cool Temperature for 96 Hours

Details

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Article

Created At

22 Jan 2023