This study was conducted to evaluate the effect of using biogen in V-line rabbit diets on its productive and reproductive performance. Twenty eight rabbits (16 does of 10 months' age averaged 3325 ± 122 g and 12 bucks of 11 months' age averaged 3440 ± 268 g live body weight were randomly assigned to four groups and fed ad libitum  as follows: T₁; Basal diet (Control), T₂, T₃ and T₄: Basal diet plus 0.5, 0.75 and 1 g biogen/Kg diet, respectively. Results showed that doe's in T₃ and T₄ gave better results for number of services per conception, litter size at weaning, litter weight, bunny weight and daily gain at birth and weaning. Mortality rate generally decreased than the control (T₁) and T₂. Parturition interval non-significantly decreased, with the lowest period being recorded for T₂. Total feed intake/parity showed significant (P<0.05) increase in biogen groups. Biogen in bucks' diets significantly (P<0.05) improved semen quality traits.Biogen significantly (P<0.05) increased hemoglobin, white blood cells, monocytes % and eosinophils % compared to the control. While red blood cells, hematocrit %, lymphocytes % and neutrophils were not significantly differed among groups. Biogen significantly (P<0.05) increased serum total protein, globulin and total cholesterol, whil significantly (P<0.05) reduced triglycerides, but non-significantly albumin and creatinine. Biogen (T₃ and T₄) improved the relative economic efficiency by 28.39 and 23.50%.In conclusion, 0.75 g biogen/Kg diet improved productive and reproductive performance of does and bucks and enhanced blood constituents as well as economic efficiency.