Alleviating the effects of transportation stress was the purpose of this study by using 270 thirty-five-day-old Ross 308 broiler chickens to check out the effects of vitamin C and acetyl salicylic acid (ASA) at different levels via drinking water. Birds in the experiment were randomly allocated to 27 identical pens (140×120×90 cm) with 10 birds each (5 males and 5 females -almost equal in weight-). At the last 24 hours of experiment pens were assigned to 9 water treatments: at levels of 0 (Control),50mg/L vitamin (T1),100mg/L vitamin C (T2), 1 gm/L salicylic acid (T3), 1.5 gm/L salicylic acid (T4), 50mg/L vitamin C with 1 gm/L salicylic acid (T5), 50mg/L vitamin C with 1.5 gm/L salicylic acid (T6), 100mg/L vitamin C with 1 gm/L salicylic acid (T7) and 100mg/L vitamin C with 1.5 gm/L salicylic acid (T8). Studied traits were: glucose, total protein, uric acid, albumin, calcium, globulin, ALT, AST, Creatine Kinase and hormones (T3 and T4) studied in this experiment. Results indicate that incorporating Vit. C and ASA into broilers water compared to control group decreasethe value of all stress indicator in a way that each of (glucose, albumin, globulin, uric acid, calcium, ALT, AST, Creatine Kinase and T3) significantly decreased.  However, T4 was significantly increased while total protein was not changed. Results concluded that supplementation of the broiler drinking water with Vitamin C and ASA before transportation can be used as effective way for alleviating transporting stress and supporting the broilers to have more stable physiology during transportation.