This study was conducted to evaluate the effect of grape juice treated with zinc in both forms [chelated zinc (CZn) and zinc oxide nanoparticles (ZnONPs)], on rabbits performance. Forty-two male rabbits were divided into seven groups (6 each). The 1^stgroup was administered 20ml/kg BW water daily (negative control).The 2^nd group was taken 20ml/kg BW untreated grape juice (without zinc) daily (positive control).The 3^rd group was treated with 20ml/kg BW grape juice that has been treated with CZn at 1.5 gL^-1 daily, While rabbits of the 4^th, 5^th,6^th, &7^th groups were taken 20ml/kg BW of grape juice that has been treated with ZnONPs at 60, 120, 240 & 480mgL^-1, respectively, daily. Results showed that growth performance, feed efficiency, serum proteins, and hematological parameters of rabbits treated with either ZnONPs or CZn were significant (P< 0.001) improved until they reached their maximum in G6 and then began to decline after that in G7. Blood glucose levels followed the same trend. Liver and kidney function continued to gradually increase with increasing ZnONPs rate. ZnONPs treatments had significantly decreased in total cholesterol and triglycerides, but high &low-density lipoprotein were not affected compared to control. ZnONPs grape juice administration had significantly  raised the serum GSH content and SOD activity compared to the control group. It can be concluded that using grape juice treated with ZnONPs up to 240mgL^-1 has no adverse effects on rabbits health. On the other hand, using the grape juice treated with ZnONPs at 480mgL^-1 has adverse effects on the rabbits health.