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153291

The Effect of Cryopreservation Temperature and Time on Viability and In Vitro Maturation of Sheep Oocytes

Article

Last updated: 22 Jan 2023

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Abstract

This study aim to compare the effects of freezing techniques on viability and in vitro maturation of sheep oocytes. This experiment was carried out in Animal Production Research Institute. Sheep ovaries were obtained from slaughterhouse. A total of 695 oocytes were used in this study, 114 oocytes as control and 581 were exposed to LN2 vapor then divided into 3 groups: G1(190); directly plunged into LN2, G2 (198): cryopreserved in ˗80 oC freezer and, G3 (193), plunged into LN2 for 10 min and then transferred to –80 oC freezer. Oocytes in each group were cryopreserved for (24h, and 7days). There were high significantly differences (P<0.01) between the percentage of damage for cryopreserved oocytes, where zona dissolution was the main damage for (G1and G3) oocytes. Whereas, zona rupture was the highest damage occurred in (G2) oocytes. Survival rate of G1 oocyte was higher significantly (P<0.05) than G3 and G2, the same results were obtained pattern, either after 24h or 7 days storage. Highly significant differences (P<0.01) between maturation rate achieved with the control oocytes (63%) compared with the other groups. After 24 h of storage, G1 recorded higher MII rate followed by G3 and G2 (47, 37 and 28%, respectively), MII % decreased at 7 days storage in G1, G3 and G2 (44%, 34% and 31%, respectively). In conclusion, Cryopreservation of sheep oocytes (G3) in –80 °C freezer achieved acceptable survival and in vitro maturation rates. The –80°C freezer provide a simple and cheap tool to maintain frozen sheep oocytes.

DOI

10.21608/jappmu.2021.62680.1010

Keywords

Ewes - Oocyte - cryopreservation- storage time, IVM

Authors

First Name

Ehab

Last Name

Abdel-Aal

MiddleName

Salah

Affiliation

Animal production, faculty of Agriculture , Cairo university

Email

ehabsal240@yahoo.com

City

Giza

Orcid

-

Volume

12

Article Issue

2

Related Issue

22566

Issue Date

2021-02-01

Receive Date

2021-03-03

Publish Date

2021-02-01

Page Start

79

Page End

83

Print ISSN

2090-3642

Online ISSN

2090-3723

Link

https://jappmu.journals.ekb.eg/article_153291.html

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https://jappmu.journals.ekb.eg/service?article_code=153291

Order

3

Type

Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023