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153290

Selenium and Zinc as Supplements to Extenders Frozen Semen for Improving Sperm Characteristics during Cryopreservation

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Last updated: 04 Jan 2025

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Abstract

Aim of this study is to evaluate the effect of adding different levels of Zinc and Selenium on extender as antioxidants to improve Friesian-bull semen quality after cryopreservation. Semen was collected, pooled, and extended with five treatment extenders, including control without (E1), 0.3 mg/100ml Zn (E2), 0.6 mg/100ml Zn (E3), 0.2 mg/100ml Se (E4), and 0.5 mg/100ml Se (E5). Semen was evaluated for visual parameters after dilution, equilibration, and thawing. Also, semen was evaluated by CASA only after thawing. Results showed that there are no effects of all additives on sperm characteristics after dilution and thawing. Only visual progressive motility percentage increased (P<0.05) post-equilibration by E5. Percentages of acrosome integrity were enhanced by E4 and E5. Semen analysis by CASA revealed that non-motility, and total and head abnormalities percentages were decreased (P<0.05), while vitality, and total and rapid progressive motility percentages were increased (P<0.05) by E5. Dynamic sperm parameters straightness and wobble) were increased (P<0.05) E5. In conclusion, adding selenium at a level of 0.5 mg/100ml to extender of cryopreserved Friesian-bull semen ha beneficial effects on maintaining sperm parameters and improved sperm freezability. This may be used as a tool for improving cryopreserved semen in artificial insemination centers.

DOI

10.21608/jappmu.2021.153290

Keywords

Friesian semen, zinc, selenium, cryopreservation, sperm dynamics

Authors

First Name

W.

Last Name

Fouad

MiddleName

F.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Egypt.

Email

waelfikry83@gmail.com

City

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Orcid

-

First Name

A.

Last Name

Ashour

MiddleName

F.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Egypt.

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City

-

Orcid

-

Volume

12

Article Issue

2

Related Issue

22566

Issue Date

2021-02-01

Receive Date

2021-03-03

Publish Date

2021-02-01

Page Start

71

Page End

77

Print ISSN

2090-3642

Online ISSN

2090-3723

Link

https://jappmu.journals.ekb.eg/article_153290.html

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https://jappmu.journals.ekb.eg/service?article_code=153290

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2

Type

Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

MainTitle

Selenium and Zinc as Supplements to Extenders Frozen Semen for Improving Sperm Characteristics during Cryopreservation

Details

Type

Article

Created At

22 Jan 2023