Knowledge of influence added more one concentration of L-arginine to enhance the poor quality of buffalo bull spermatozoa considers the main objective in our study. Semen collected from five buffalo bulls once weekly and ejaculates with 45-50% mass motility were pooled. Semen was diluted at 37C° by Tris-egg yolk extender, also separated to five portions, 1^st portion without supplement it consider control to other portions  and 2^nd, 3^rd, 4^th and 5^th were supplemented with 3, 5, 7 and 10 mM L-arginine respectively. Progressive sperm motility, live sperm%, abnormal sperm%, chromatin normality, Conception rate, and integrity of sperm acrosome were determined. Data declared the, extenders with concentrations of 7 and 10 mM L-Arginine was improved all parameters studied than 3 and 5 mM L-arginine extenders and control extender at different cryopreservation processes. A significant difference in chromatin integrity was detected at all treatments. Also, the conception rate was higher in buffalo cows artificially inseminated with 7 and 10 mM L-arginine (67.27 and 71.70 %) compared with control (45.45%). Conclusively, it could be concluded that adding L-arginine with concentrations of 7 or 10mM to Tris-extender improved the cryopreservation and fertility of the poor quality of Buffalo bull spermatozoa.