This study was conducted to evaluate the effect of dietary supplementation of dairy cows with yeast culture (YC) on productivity and reproduction of primi-parous cows. Animals in the 1^st group were fed the control ration (G1), while those in G2 and G3 were fed control ration with 20 and 40 g/h/d yeast culture (n=5 in each) during experimental period of 90 days prepartum and 90 days postpartum. Results show that CF digestion pre- and post-partum, body weight (30 and 90-d postpartum), body condition score (one-d prepartum and 90-d postpartum), daily actual and 4% FCM milk yield, milk fat, total solids and solids not fat contents were higher (P<0.05) in G2 and G3 than in G1, being the highest in G3. Uterine involution was earlier (P<0.05) by 7.6 d and calf performance parameters was better in G3 than in G1. Postpartum 1^st estrus interval, days open, P4 at estrus, service period and number of services/conception reduced (P<0.05) in G3 with best conception rate. Serum immunoglobulins (IgG, IgM and IgA) at 0 and 24 h post-calving, RBCs and WBCs counts, hemoglobin, hematocrit, and serum biochemicals in cows and their calves in G3 were the highest (P<0.05), whilst ALT and AST activities were the lowest in cows and calves in G3. The current study may conclude that dietary supplementation pre- and post-partum with yeast culture (40 g/h/day) can enhance productive and reproductive performances of primi-parous cows, and immune response, healthy status of cows and their offspring.