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95834

In Vivo and In Vitro Dromedary Camel Embryos’ Production under Egyptian Conditions

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Last updated: 22 Jan 2023

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Abstract

The aim of the present study was to compare between in vivo and in vitro production of dromedary camel embryos under Egyptian conditions. At in vivo experiment, three female camels (donors) were treated with a one-shot injection of 3000 IU PMSG, followed by induction of ovulation treatment (i.m. administration of 5000 IU hCG). Natural mating was performed after 8 days from the superovulation treatment. Embryos were flushed via non-surgical collection after eight days of animal mating. For in vitro experiment the oocytes were retrieved by slicing ovaries collected from the slaughterhouse. Recovered oocytes were examined before and after IVM culture for grading and investigating cytoplasmic maturation. Only good-quality oocytes were selected (n=72).  Cumulus oocytes complexes (COCs) were incubated at 38.5 ºC, 5% CO2 and 95% humidity for 40 hours. Matured COCs were inseminated with frozen-thawed semen (3x106 spermatozoa/mL) in the fert-TALP medium at 38.5 ºC, 5% CO2 and 95% humidity for 18 hours. Results showed that the in vivo embryos recovery rate was 74.92%. The hormonal treatment had no significant effect on the activity of ovaries regarding ovarian side. For in vitro experiment the mean oocyte yield was 9.83 COCs per ovary and the recovery rate (%) was 37.5%. The maturation rate of dromedary oocytes in vitro was 72.72%, while the fertilization rate was 11.5%. In conclusion, this study showed that in vivo embryo production can be considered as an effective tool for embryo in dromedary camels when compared to embryo production in vitro.

DOI

10.21608/jappmu.2020.95834

Keywords

camels, dromedary, embryo production, IVF, IVM, oocyte, superovulation, PMSG

Authors

First Name

Amira

Last Name

Khattab

MiddleName

Kh.

Affiliation

Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt. / Embryology Department, Faculty of Science, El- Azhar University, Egypt.

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Orcid

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First Name

Nehal

Last Name

Abu Elnaga

MiddleName

A.

Affiliation

Embryology Department, Faculty of Science, El- Azhar University, Egypt.

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Orcid

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First Name

Kh.

Last Name

El-Bahrawy

MiddleName

A.

Affiliation

Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt.

Email

bhrawi@yahoo.com

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Volume

11

Article Issue

4

Related Issue

13079

Issue Date

2020-04-01

Receive Date

2020-06-14

Publish Date

2020-04-01

Page Start

155

Page End

161

Print ISSN

2090-3642

Online ISSN

2090-3723

Link

https://jappmu.journals.ekb.eg/article_95834.html

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https://jappmu.journals.ekb.eg/service?article_code=95834

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5

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Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

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Article

Created At

22 Jan 2023