The objective of this study was to estimate the genetic and phenotypic trend of total milk yield (TMY) of Egyptian buffalo. A total number of 3005 test day milk records for 401 Egyptian buffaloes, daughters of 54 sire covered the period from 1991 to 2002 were used in this study.  Data were collected from Mahllet Mousa and El- Nattaf El-Kadeem farms in Kafr El-Sheikh. The two farms belong to Animal Production Research Institute, Ministry of Agriculture and Land Reclamation, Egypt. The data were analyzed using the Least squares technique to examine the effects of year-season of calving, herd and parity on total milk yield. The effects of year-season of calving, herd, and parity on TMY were all highly significant (P>0.001). The average TMY was 1429 Kg. The heritability estimate of TMY was 0.25.  The regression of TMY on year of calving showed a positive trend (+67 kg per year of calving). Also, genetic trend was positive (+12.55kg per year of birth). In conclusion, although the trend in the estimated breeding values was positive, it still did not lead to adequate change required in the average milk production due, probably, to inadequate feeding and/or impaired management of the herds.