This work was carried out to evaluate the alleviation ability of Nigella sativa (Ns) and or vitamin C for the toxic effect of aflatoxin B₁ in rabbits diet. Forty New-Zealand White male rabbits (average body weight 1000 ± 10 g) were used in five experimental groups (8 / group) for 6 weeks. The control group (T₁) fed control diet, 2^nd group (T₂) fed the diet with 200 ppb aflatoxin B₁. The 3^rd, 4^th and 5^th group (T₃, T₄ & T₅) fed diets with 200 ppb aflatoxin B₁ plus 1% Ns, 500 mg vitamin C/ kg diet and 1% Ns plus 500 mg vitamin C/kg diet, respectively. Results showed that Ns or vitamin C addition can alleviate the negative effect of aflatoxin B₁ on internal organs weight and histopathological lesions of liver and kidneys.