This study was designed to investigate the inclusion effect of humic acid (HA) and mannanoligosaccharide (Bio-Mos) into laying hen diets on egg production and egg quality. Gimmizah layers (n=210) 30 wk of age were fed a control diet , 0.1 , 0.2 and 0.3% humic acid and 0.1 , 0.2  and 0.3 % Bio-Mos for 90 days . Feed consumption was measured weekly and feed conversion was calculated.. Mortality was recorded daily. The number of eggs and egg weight were recorded daily throughout the experimental period. Also a sample of 30 eggs from each group was collected randomly to determine egg quality every 30 days. The results were summarized as follow: egg weight and egg   production for hens supplemented with humic acid and Bio-Mos were significantly increased compared with the control group. Dietary treatments had insignificant effect on feed intake. No significant effect of humic acid or Bio-Mos had insignificantly effect  on egg shape index, albumin (%), Haugh unit and egg yolk index. Whereas, egg shell thickness was significantly (p<0.05) increased compared with the control group. In conclusion, supplementation of humic acid and Bio-Mos during laying period significantly increased egg production, egg weight and improved egg shell quality.