The present study aimed to investigate the effect of total glycoalkaloid (TGA ) (solanine and chaconine) in potato by-products hay on nitrogen retention, semen production capacity using partial depletion of epididymis and cryoportectant ram sperm characteristics and incubation after thawing. Six rams with an average body weight of 70. kg and 3 years of age were used and assigned randomly into two similar groups. The supplemented feeding ratio was 50% concentrate feed mixture as main diet for two experimental ram groups and 50% of treatment rations (berssem hay, potato by-products hay). Berseem hay was supplemented as control group and second group was nourished offered to potato by-products hay (PB-PH). Nitrogen balance and nutrients digestability were determined for experimental groups and studying sperm characteristics which collected three consecutive ejaculates daily up to fourteen successive days using a warm artificial vagina with estrous ewe for mounting, liquid nitrogen for sperm incubation period through three hours was determined with post thawing spermatozoa. The obtained results indicated that the rams fed the ration contained PB-PH were significantly (P<0.05) lower in experimental testes (nitrogen retention, and thawing sperm characteristics during incubation time at 370C for 3 hours) than rams given BH ration . The overall means of total motile, normal and concentration number sperm / ejaculates x109 were 1.31, 1.47 and 1.75, with PB-PH ration while control group recorded 1.63,1.75 and 1.99,respectively during epididymis depletion. The PB-PH post-thawing sperm motility, live and normal were 43.00%, 40.30% and 44.00%,respectively. time at 370C for 3 hours) than control group. The incubation post-thawing sperm characteristics for deferent experimental rations and incubation time three hours were highly significant (P<0.05). The overall means of PB-PH treatment were 31.25%, 28.72% and 32.52% while, control were 39.38 , 33.88 and 40.26 for post-thawing motility, live and normal, during incubation respectively. Sperm characteristics decline through the incubation times because of lactic dehydrogenase (LDH), lactic acid and aromatic amino acid oxidase (AAAO) releasing gradually.